Column Watch: Methods for the Passivation of HPLC Instruments and Columns
نویسندگان
چکیده
Metal-analyte interaction is a type of nonspecific adsorption (NSA) that occurs mainly between acidic analytes and active adsorptive sites present in the metal surfaces fluidic path any chromatographic system. NSA represents challenge since it may lead to poor peak shape, low recovery, performance from high liquid chromatography (HPLC) As result, many solutions circumvent mitigate this secondary have been published or commercialized last few years. Such encompass swapping stainless steel components for non-reactive polymers, “biocompatible” metals alloys, addition additives into mobile phase, pretreating system with large amounts sample strong acids, use coated materials. This article explores these more detail, particularly recent adaptation benefits LC.
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ژورنال
عنوان ژورنال: Lc Gc North America
سال: 2023
ISSN: ['1527-5949']
DOI: https://doi.org/10.56530/lcgc.na.pj7690e5